Applications:Suitable for use in Immunoblot, ELISA, Immunohistochemistry, Immunomicroscopy.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:80,000 in a standard capture ELISA using peroxidase conjugated Streptavidin (S7973-97A) and ABTS as a Substrate for 30 min at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.
Specificity：Recognizes all Human immunoglobulin subclasses, isotypes and chain combinations. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-goat serum.
