Applications:Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:50,000.
Western Blot: 1:1,000-1:5,000Immunohistochemistry: 1:500-1:3,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with HRP.
Specificity：Recognizes human IgA alpha. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgA and human serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.
