Applications:Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:30,000-1:150,000.
Assayed against 1ug of Human IgG in a standard capture ELISA using ABTS (A0030) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with Horseradish Peroxidase (HRP).
Specificity：Recognizes human IgG F(ab’)2. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG, human IgG F(ab’)2 and human serum. Does not crossreact with human IgG F(c).
