Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry, and Immunomicroscopy.
Other applications not tested.
Recommended Dilution:ELISA: 1:60,000 to 1:250,000, Assayed against 1ug of human IgG in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 min at RT
Optimal dilution determined by the researcher.
Biotin/Protein Ratio: 10–20 BAC molecules per Goat IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.6, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) Thimerosal, 40% glycerol.
Specificity：Recognizes human IgG F(ab’)2 fragment. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti goat serum, human IgG, human IgG F(ab’)2 and human serum. No reaction was observed against human IgG F(c).
