Applications:Suitable for use in FLISA, Western Blot and Immunofluorescence.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:6.6 moles FITC per mole of Goat IgG
Storage and Stability:Lyophilized powder may be stored at -20°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, dH2O and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile 40-50% glycerold, ddH2O.
Specificity:Recognizes human IgM (mu chain). Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgM and human serum. No reaction was observed against human IgG or human IgA. Specificity was confirmed by ELISA at < 1% crossreactivity against other human heavy or light chain isotypes.