Applications:Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase electron microscopy, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:70,000.
Western Blot: 1:1000- 1:5000
Immunohistochemistry: 1:500- 1:2500
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizeshuman IgG Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(ab’)2.