Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:20,000-1:100,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10–20 BAC molecules per goat IgG molecule
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.
Specificity：Recognizes human IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(ab’)2.
