Applications:Suitable for Western Blot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:2000-1:10,000ELISA: 1:10,000-1:100,000Immunohistochemistry: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (Protease and IgG free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum. Specificity was confirmed by ELISA, ≤1% crossreactivity against other human heavy or light chain isotypes.
