Applications:Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:80,000; 1.0ug of Human IgG was used in a standard capture ELISA with Streptavidin (HRP) (S7973-97A) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) (A0030) as a substrate for 30 minutes at RT
Optimal dilution determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per Goat IgG molecule
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 24 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes human IgG. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-goat serum, human IgG and human serum. No reaction was observed against bovine, chicken, goat, guinea pig, hamster, equine, mouse, rabbit, rat and sheep serum proteins.
