Applications:Suitable for use in Immunofluorescence and FLISA (Fluorescent) (Fluorescent).
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:1000-1:5000FLISA (Fluorescent): 1:10,000-1:50,000Optimal dilution determined by the researcher.
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile, ddH2O. Fluorochrome/Protein Ratio: 3.4 moles FITC per mole of Goat IgG.
Specificity：Recognizes human IgG. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, human IgG and human serum. No reaction was observed against bovine, chicken, goat, guinea pig, hamster, equine, mouse, rabbit, rat and sheep serum proteins.
