Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit IgG moleculeApplication(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilution(s):ELISA: 1:20,000 to 1:100,000.
Assayed against 1ug of goat IgG in a standard capture ELISA using peroxidase conjugated streptavidin.
A working dilution of of the reconstitution concentration is suggested for this product.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：rabbit
Source Antibody：goat
Grade：Affinity Purified
Purity：Prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a liquid in 0.02 M potassium phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% (w/v) thimerosal, 25% glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Goat IgG, Goat IgG F(c) and Goat Serum. No reaction was observed against Goat IgG F(ab’)2
