Applications:Suitable for use in Immunomicroscopy, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.Other applications not tested.
Recommended Dilution::ELISA: 1:600,000Western Blot: 1:60,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10–20 Biotin molecules per Donkey IgG molecule
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：donkey
Source Antibody：goat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes Goat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-donkey serum, goat IgG and goat serum. No reaction was observed against chicken, guinea pig, equine, hamster, mouse, rabbit and rat serum proteins.
