Applications:Suitable for use in Immunofluorescence and FLISA.
Other applications have not been tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Fluorchrome/ Protein Ratio:3.9 moles FITC/mole Donkey IgG.
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Label:Fluorescein isothiocyanate (FITC).
(Molecular weight 390D).
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:donkey
Source Antibody:goat
Grade:Affinity Purified
Purity:Purified monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Conjugated with FITC.
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, equine, Mouse, Rabbit and Rat Serum Proteins. Minimum Crossreactivity to Chicken, Guinea Pig, Hamster, equine, Mouse, Rabbit and Rat Serum Proteins