Applications:Suitable for use in Immunofluorescence, FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:10,000-1:50,000Immunofluorescence: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Excitation Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:3.7
Storage and Stability: :Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1mg
Host:donkey
Source Antibody:goat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC)
Specificity:Recognizes goat IgG, H&L. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-donkey serum, goat IgG and goat serum.