Applications:Suitable for use in Immunofluorescence, Western Blot and FLISA.
Other applications not tested.
Recommended Dilution:Immunoflourescence Microscopy: 1:1000-1:5000FLISA: 1:10,000-1:50,000Optimal dilution determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:3.5 moles FITC per mole of Rabbit IgG
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
仕様
Size:1mg
Host:rabbit
Source Antibody:canine
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.6, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:Recognizes canine IgG, F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, canine IgG, canine IgG F(ab’)2 and canine serum. No reaction was observed against canine IgG F(c).