Applications:Suitable for use in Immunoperoxidase Electron Microscopy, ELISA, Western and Dot Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000-1:72,000 Assayed against 1ug of canine IgG using ABTS (A0030) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2mg
Host：rabbit
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. before the addition of glycerol to 40%. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes Canine IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, canine IgG and canine serum.
