Applications:Suitable for use in Immunomicroscopy, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:4500-1:8000Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size：1mg
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, pH 8.0, 10mg/ml BSA, (IgG and Protease free) 0.09% sodium azide, 50% glycerol. Labeled with alkaline phosphatease (AP).
Specificity：Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, mouse IgM and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
