Applications:Suitable for use in FLISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:FLISA 1:10,000-1:50,000Immunofluorescence: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:3.7 moles FITC per mole of Goat IgG
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography and solid phase adsorption to remove any unwanted crossreactivities.
Form：Supplied as a liquid in 0.02M PBS, pH 7.2, 0.15M sodium chloride, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity：Recognizes mouse IgM mu heavy chain. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgM and mouse serum. Does not crossreact with mouse IgG or mouse IgA. Exhibits less than 1% crossreactivity with other mouse heavy or light chain isotypes.
