DTAF has absorption and emission properties nearly identical to fluorescein isothiocyanate (FITC) and DTAF- and FITC-labeled antibodies are similar in terms of preparation and specificity of immunofluorescence.
However, DTAF is superior to FITC with regard to cost, purity and stability.
Also, DTAF-labeled rabbit IgG conjugates can be quickly and efficiently fractionated by simple ammonium sulfate precipitation to yield preparations free of both over- and under-conjugated material.
In addition, over-conjugated protein can be readily removed from DTAF:IgG conjugates by appropriate adjustment of pH and temperature.
Applications:Suitable for use in FLISA, Immunofluorescence and Fluorescent Western Blot.
Other applications not tested.
Recommended Dilutions:FLISA: 1:10,000-1:50,000Immunofluorescence: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Label (FITC):Absorption Wavelength: 495nmEmission Wavelength: 528nmFluorochrome/Protein Ratio: 3 moles FITC per mole of IgG
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC.
Specificity：Recognizes mouse IgG, F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgG, mouse IgG F(ab’)2 and mouse serum. No reaction was observed against mouse IgG F(c).
