Applications:Suitable for use in FLISA and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Label: Fluorescein isothiocyanate (FITC)Molecular Weight: 390DAbsorption Wavelength: 495nmEmission Wavelength: 528nmFluorochrome/Protein Ratio: ~3 moles FITC per mole of Goat IgG
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile dH2O or 40-50% glycerol for long term storage.
Specificity:Recognizes Mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgG and mouse serum. Species Crossreactivity: Minimum Crossreactivity to bovine, chicken, goat, guinea pig, hamster, equine, human, rabbit, rat and sheep serum proteins