Applications:Suitable for use in Immunomicroscopy, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:2000-1:8000Western Blot: 1:500-1:4000Immunohistochemistry: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 0.01% sodium azide, 10mg/ml BSA (IgG and Protease free). Labeled with AP
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. Minimum Crossreactivity to Human Serum Proteins.