Applications:Suitable for use in Immunomicroscopy, ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:80,000 to 1:320,000 using peroxidase conjugated streptavidin and ABTS as a substrate for 30 min.
at RT
Optimal dilution determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per goat IgG molecule.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied in PBS, pH 7.2, 0.01% sodium azide, 10 mg/ml BSA (IgG and protease free), 40% glycerol. Labeled with biotin.
Specificity:Recognizes Mouse IgG, whole molecule. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, mouse IgG and mouse serum.