Applications:Suitable for use in Immunofluorescence, FluorescentFLISA (Fluorescent) (Fluorescent) (FLISA (Fluorescent)) and Fluorescent Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunofluorescence: 1:100-1:5000FLISA (Fluorescent): 1:10,000-1:50,000Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:3.5 moles FITC per mole of Goat IgG
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light.
仕様
Size:1mg
Host:goat
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes mouse IgG. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgG and mouse serum.