Applications:Suitable for use in Immunomicroscopy, ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:50,000-1:200,000; Assayed against 1ug of mouse IgG in a standard capture ELISA using Streptavidin-HRP and ABTS as a substrate for 30 min.
at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio:10-20 BAC molecules per Donkey IgG molecule
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：donkey
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA, 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml deionized water containing 40-50% glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-donkey serum, mouse IgG and mouse serum. No reaction was observed against bovine, chicken, equine, goat, guinea pig, hamster, human, rabbit, rat and sheep serum proteins.
