Applications:Suitable for use in ELISA, Immunoblot (Western or Dot Blot), Immunoperoxidase electron microsccopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:70,000Western Blot: 1:2000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：donkey
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-donkey serum, mouse IgG and mouse serum.
