Applications:Suitable for use in Immunofluorescence, Fluorescent Western Blot and FLISA (Fluorescent).
Other applications have not been tested.
Recommended Dilutions:Immunofluorescence: 1:1000-1:5000FLISA (Fluorescent): 1:10,000-1:50,000Optimal dilution determined by the researcher.
Excitation Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio:3.5 moles of FITC/mole of chicken IgG
Recommended Secondary Antibodies: I1906IgY, Chicken Pab Rb x Ch I1906-10IgY, Chicken (HRP) Rb x Ch I1906-12IgY, Chicken (FITC) Rb x Ch I1906-14IgY, Chicken (AP) Rb x Ch
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light.
仕様
Size：1mg
Host：chicken
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-chicken serum, mouse IgG and mouse serum.
