Applications: Suitable for use in Western Blot, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilution:ELISA: 1:28,000-1:116,000 using ABTS as substrate detects 1ug of Goat IgG in standard sandwich ELISA..
Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：goat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, goat IgG, anti-biotin and goat serum.
Form：Supplied as a lyophilized powder or liquid in PBS, pH 7.2, BSA, 0.01% sodium azide. Labeled with Biotin.
Specificity：Recognizes goat heavy and light chains. No reaction was observed against human serum proteins.
