Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.
TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions.
Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:ELISA: Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.15-0.30ug/ml of antibody.
This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2–0.4ng/well.
Western Blot: Use at a concentration of 0.1–0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit is 1.5–3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS containing 0.1% BSA..
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Country of Origin: USA
仕様
Size：25ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immobilized antigen affinity chromatography.
Form：Supplied as a lyophilized powder in PBS. Labeled with Biotin.
