Applications:Suitable for use in ELISA and Western Blot.
Others applications not tested.
Recommended Dilutions:ELISA (Direct): ~1ug/ml (using 100ul/well antibody solution) This antibody, in conjunction with compatible secondary reagents, allows detection of 0.2–0.4ng/well of recombinant mouse MIP-1y.
ELISA (Sandwich): 0.25-1ug/ml (using 100ul/well antibody solution) Allows detection of 0.2–0.4ng/well of recombinant mouse MIP-1y.
Western Blot: 0.1–0.2ug/ml.
The detection limit is 1.5–3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determinded by researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS, 01% BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：25ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immobilized antigen affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 50ul of sterile PBS containing 01% BSA. Labeled with Biotin.
