Human GCP-2 is an 8kD protein containing 73 amino acid residues.
Neutrophil recruitment to inflammatory sites is mediated by two related receptors: CXCR1 and CXCR2.
Both receptors share two ligands, interleukin-8 (CXCL8) and GCP2 (CXCL6).
GCP2 belongs to the intercrine alpha (chemokine CxC) family.
GCP-2 promotes neutrophil chemotaxis and degranulation.
Application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGCP-2 (100.0 ng/ml), a concentration of 2.0–5.0 ug/ml of this antibody is required.
ELISA: To detect hGCP-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5ug/ml of this antibody is required.
This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2–0.4 ng/well of recombinant hGCP-2.
Western Blot: To detect hGCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1–0.2 ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hGCP-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Usage: For research use only.
Reconstitution: The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at- 20°C.
When reconstituted in sterile water to a concentration of ≥ 0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C.
Country of Origin: USA
仕様
Size：25ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by antigen affinity chromatography.
Form：Supplied as a lyophilized powder from a sterile-filtered solution in PBS, pH 7.4. Labeled with Biotin.
