Applications:Suitable for use in FLISA and Immunofluorescence and Fluorescent Western Blot.
Other applications not tested.
Recommended Dilutions:FLISA: 1:10,000-1:50,000Immunofluorescence: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:3.3 moles FITC per mole of Rabbit IgG
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ul
Host:rabbit
Source Antibody:sheep
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free). 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes sheep IgG (H&L). Immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, sheep IgG and sheep serum.