Cells respond to heat and chemical stress by inducing the synthesis of a group of proteins referred to as heat shock proteins.
HSP90 is present in cells at high levels in the cytoplasm and is composed of two separate gene products, HSP90a and HSP90b.
Recently, a cocktail of HSP90a and HSP90b polyclonal antibodies was shown useful in identifying node-negative breast cancer patients at higher risk of tumor recurrence.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Suggested Dilutions:Immunoprecipitation (Native): 10ug/mg protein lysate (Use Protein A).
Western Blot: 5-10ug/ml for 2hrs at RT
Immunohistochemistry (Frozen & formalin/paraffin): 5-10ug/ml for 30 min at RT.
Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Optimal dilutions to be determined by the researcher.
Control Peptide: H1832-80
Positive Control: MAD109 cells or breast carcinoma
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4 Also supplied with BSA and sodium azide. See H1832-81.
Specificity：Recognizes Heat shock protein 90a (HSP90a). Weakly crossreacts with hsp90b/hp84. Species crossreactivity: Human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to aa 2-12 (PEETQTQDQPM-Cys) from the N-terminus end of mouse heat shock protein 90a (HSP90a). MW of Antigen: 86kD. Cellular Localization: Cytoplasmic and nuclear.
