Several peptides associated with feeding behavior have been reported recently.
Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus and are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.
Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains.
The expression was detected in brain and to a small extent in testis.
These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs).
Rat and human Orexin 1Receptor (Hypocretin 1 Receptor) are 416 aa, and 425aa, respectively.
Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000 (Use control peptides to confirm antibody specificity)Western Blotting: 1-10ug/ml using chemiluminescenceImmunohistochemistry: 2-20 ug/mlAntigen retrieval was carried out by pressure cooker unmasking for 2 min in 0.01 M sodium citrate buffer (pH 6.0)
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by antigen affinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity：Rat Orexin 1 Receptor (Hypocretin 1 Receptor) is 82% homologous with human. The appropriate control immunogenic peptides may be used to confirm specificity of antibodies. Antibody crossreactivity in various species is not established.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptides, 16 aa, near the N-terminus of rat Orexin 1 Receptor (Hypocretin 1 Receptor) and a 16 aa peptide near the C-terminus of human.
