Several peptides associated with feeding behavior have been reported recently.
Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus and are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.
Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains.
The expression was detected in brain and to a small extent in testis.
These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs).
Rat and human Orexin 1Receptor (Hypocretin 1 Receptor) are 416 aa, and 425aa, respectively.
Applications: Suitable for use in ELISA, Western Blotting.
Recommended Dilution:ELISA: 1:10,000-1:1000 (Use control peptides to confirm antibody specificity)Western Blotting: 1:1000-1:100,0 (use 50–100ng of control peptide/well; control peptides, because of their low MW (< 3kD), are not suitable for Western blot.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Neat serum
Form：Supplied as neat serum with preservative.
Specificity：Rat Orexin 1 Receptor (Hypocretin 1 Receptor) is 82% homologous with human. The appropriate control immunogenic peptides may be used to confirm specificity of antibodies. Antibody crossreactivity in various species is not established.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptides, 16 aa, near the N-terminus of rat Orexin 1 Receptor (Hypocretin 1 Receptor) and a 16 aa peptide near the C-terminus of human.
