Prepro-orexin is 130 amino acid long peptide with a putative 33 AA secretory sequence, a hydrophobic core followed by residues with small polar side chains.
The expression was detected in brain and to a small extent in testis.
These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R..
Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus.
Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.
Applications:Suitable for use in ELISA.
Other potential application, though not tested is Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:10,000-1:1000 using 50-100ng of control peptide/well
Optimal dilution determined by the researcher.
Control Peptide:O7502-10: Orexin Prepro, Control Peptide (Hypocretin Prepro)
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyopohilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes mouse Orexin A Pre-pro. The sequence is not found in Orexin A or Orexin B. Will not recognize mature Orexin A or Orexin B.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to 17aa near the C-terminus of mouse prepro-Orexin, conjugated to KLH. Species Sequence Homology: rat; 82%, human; 70%.
