Prepro-orexin is 130 amino acid long peptide with a putative 33aa secretory sequence, a hydrophobic core followed by residues with small polar side chains.
The expression was detected in brain and to a small extent in testis.
These neuropeptides bind and activate two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R..
Orexins (Orexin A and Orexin B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus.
Orexin A and Orexin B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000; Use 50-100ng of control peptide/well
Western Blot: 1-1000-1:5000l using ECL
Optimal dilution determined by the researcher.
Control Peptide:O7502-10
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Neat serum
Form：Supplied as a lyophilized powder with 0.05% sodium azide.
Specificity：Recognizes mouse Prepro Orexin. Will not detect mature Orexin A or Orexin B. Species Sequence Homology: Rat - 82% (14/17aa), Human - 70% (12/17aa)
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide, 17aa, corresponding to the carboxy terminus of murine prepro-Orexin. The sequence is not found in Orexin A or Orexin B.
