Hypothalamic neuropeptides associated with feeding behavior, binding and activating two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000.
(use 50-100ng of control peptide/well)
Western Blot: 1-10ug/ml using Chemiluminescence technique
Optimal dilution to be determined by the researcher.
Control Peptide:O7501-10: Orexin B, Mouse, Control Peptide
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity：Recognizes full-length (1-28aa) mouse Orexin B. The epitope is not found in Orexin A. Since Orexin B are derived from an unprocessed pre-pro orexins (130aa), the antibody may also react with prepro orexins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide, 13aa corresponding to N-terminus of murine Orexin B (KLH). Species sequence homology: mouse, porcine, bovine, canine and rat- 100%, human- 92%.
