Hypothalamic neuropeptides associated with feeding behavior, binding and activating two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.
Applications:Suitable for use in ELISA.
Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:1000.
Use 50-100ng of control per wellOptimal dilution to be determined by the researcher.
Control Peptide:O7500-10: Orexin A, Mouse, Control Peptide (Hypocretin A).
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by antigen affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity：Recognizes the full-length (1-33 aa) of mouse Orexin-A. The epitope is not found in Orexin B. Since Orexin A and Orexin B are derived from an unprocessed pre-pro-orexin (130 aa), the anti-Orexin A antibodies may also react with prepro orexins.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide, 18aa of mouse Orexin A (KLH coupled). Epitope: N-terminus. Species sequence homology: rat, porcine, ovine, bovine, canine, human 100% and chicken 81%.
