Western Blot, though not tested, may potentially be used as an application.
Other applications not tested.
Recommended Dilution:ELISA: 1:10,000-1:100,000.
Use 50-100ng of control peptide/well
Western Blot: 1:1000-1:5000.
Control peptide (O7500-10), because of low MW (< 3kD), is not suitable for Western
Optimal dilutions to be determined by the researcher.
Control Peptide: O7500-10
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Serum
Purity：Neat serum
Form：Supplied as a lyophilized powder in 0.05% sodium azide. Reconstitute with 0.1ml sterile, ddH2O or sterile 40-50% glycerol.
Specificity：Recognizes the full-length (1-33aa) Orexin-A. The epitope is not found in Orexin B. Since Orexin A and Orexin B are derived from an unprocessed pre-pro-orexin (130aa), the anti-Orexin A antibodies may also react with prepro orexins. Species Sequence Homology: rat, porcine, sheep, bovine, canine, human: 100%; chicken: 81%.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Mo
Immunogen：An 18aa synthetic peptide mapping at the N-terminus of murine Orexin A (KLH coupled).
