Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA : 1:600,000Western Blot : 1:2,000 - 1:10,000Immunohistochemistry: 1:1,000 - 1:5,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:chicken
Grade:Affinity Purified
Purity:Purified monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat or Sheep Serum Proteins.
Form:Supplied as a liquid in PBS, pH7.4, 0.01% sodium azide, BSA.
Specificity:Recognizes chicken IgY. Minimum Crossreactivity to Bovine, Goat, Guinea Pig, Hamster, equine, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins