Applications:Suitable for use in Immunoperoxidase Electron Microscopy, ELISA, Immunoblot (Western or Dot Blot) and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Other applications not tested.
Recommended Dilution:ELISA: 1:50,000Western Blot: 1:2000-1:20,000Immunohistochemistry: 1:1000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:goat
Source Antibody:chicken
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied in PBS, pH 7.2, sodium chloride,10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, before the addition of glycerol to 40%. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes chicken IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, chicken IgG and chicken serum.