Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), is an 18.7kD protein that shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI.
The reported biological activities of IL-10, which may be inter-related, include inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production.
Applications:Suitable for use in Neutralization, Sandwich ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Sandwich ELISA: 0.5-2ug/ml to detect rat IL-10 using 100ul/well antibody solution.
Used in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant rat IL-10
Western Blot: 0.1-0.2 ug/ml.
Used in conjunction with compatible secondary reagents, the detection limit for recombinant rat IL-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Country of Origin: USA
仕様
Size：50ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2.
Specificity：Recognizes rat Interleukin-10.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Rt
Immunogen：Highly purified (>98%), recombinant rat Interleukin-10, expressed in E. coli
