Human Hemofiltrate CC chemokine-1 (HCC-1) was first isolated from the hemofiltrate of patients with chronic renal failure.
HCC-1 has a 46% amino acid sequence identity with MIP-1a/MIP-1b and 29-37% homology with other CC chemokines.
Human HCC-1 is an 8.4kD protein containing 72 amino acid residues.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution In conjunction with biotinylated anti-human Hepatocellular Carcinoma (H1999-05A) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human Hepatocellular Carcinoma
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human Hepatocellular Carcinoma is 1.5-3ng/lane, under either reducing or non-reducing conditions
Immunohistochemistry (FFPE): 1.5ug/ml with an overnight incubation at 4ºC.
An HRP-labeled polymer detection system was used with a DAB chromogen.
Proteinase K antigen retrieval is recommended
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: <0.1ng/ug of protein (<1EU/ug)
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes human Hepatocellular Carcinoma.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Hepatocellular Carcinoma expressed in E. coli.
