Rotavirus is a virus that infects the bowels and is the most common cause of gastroenteritis in infants.
7 major groups have currently been identified, 3 of which infect humans (groups A, B and C).
Its genome consists of 11 segments of double stranded RNA held in the inner core of the three layered, icosahedral virion particle.
The genome codes for 6 virus proteins (VP1,2,3,4,6,7) and 6 non-structural proteins (NSP1-6).
Outer capsid proteins are VP4 and VP7, inner capsid -VP6 and core -VP2.
The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection.
Applications:Suitable for use in Immunofluorescence.
Direct FA staining of target antigens in a permissive tissue culture system.
Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.
Titer:Indirect Immunofluorescence: ~1:250
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：goat
Grade：Highly Purified
Purity：Purified by chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes ICPs and late structural (virion) antigens. Reacts >90% with human rotaviruses. Uninfected Cell Reactivity: Negative vs. HEp-2 cells and WI-38 cells by indirect immunofluorescence.
