Chlamydia trachomatis, an obligate intracellular human pathogen, is one of three bacterial species in the genus Chlamydia.
C.
trachomatis is a Gram-negative bacteria, therefore its cell wall components retain the counter-stain safranin and appear pink under a light microscope.
In humans both sexes can display urethritis, proctitis (rectal disease and bleeding), trachoma, and infertility following infection with Chlamydia trachomatis.
Applications:Suitable for use in Immunofluorescence, FLISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Acetone fixation is recommended
Optimal dilutions to be determined by the researcher
Titer:Immunofluorescence: >1:1000 by indirect Immunofluorescence vs.
all serovars (A-K, L1-L3)
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:1ml
Host:goat
Grade:Highly Purified
Purity:Chromatographically purified.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes the Major Outer Membrane Protein of Chlamydia trachomatis. Recognizes the following serovars: A, B, Ba, C, D, E, F, G, H, I, J, K, L1, L2, L3. Does not react with C. psittacii or C. pneumoniae in MIF. Uninfected Cell Reactivity: Negative vs. HEp-2 cells and egg yolk sac.
Isotype:IgG
Calc Applications Abbrev:FLISA IF IHC
Immunogen:Chlamydia trachomatis, Major Outer Membrane Protein from strain L2.