84-1016-55　Factor VIII Related Antigen (vWF, Factor VIII Related Antigen) 500ul　F0016-13

特徴

• This protein has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin.
• von Willebrand factor is synthesized by endothelial cells and stored in the Weibel-Palade granules.
• It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.
• Antibody reacts specifically with the endothelial cells of normal, reactive, and neoplastic blood and lymphatic vessels and shows a finely granular cytoplasmic staining.
• It also reacts with endocardium, platelets, and megakaryocytes.
• von Willebrand factor is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms.
• However, because not all endothelial cells synthesize (or store) this molecule, about 30% of tumors of vascular origin fail to stain for factor VIII related antigen, regardless of whether they are benign or malignant.
• Staining for factor VIII related antigen has also been used to measure angiogenesis which reportedly predicts tumor recurrence.
• Applications:Suitable for use in Immunofluorescence and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Immunohistochemistry (formalin/paraffin): 1:80-1:160 for 20 min at RT.
• Staining formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Optimal dilutions to be determined by the researcher.
• Positive Control:Tonsil, Placenta
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.