c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR.
C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors.
Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer.
Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy.
Applications:Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 5-10ug/ml for 2 hours at RTImmunoprecipitation: 10ug/mg protein lysate (Use protein A)Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes
Optimal dilutions to be determined by the researcher.
Positive Control:SKBR-3 cells, T47D cells or breast carcinomas.
Control Peptide:C0015-30: c-erbB2, aa1243-1255, Control Peptide (HER2, neu Receptor)
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA.
Specificity：Recognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Species Crossreactivity: monkey, rat and mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide derived from C-terminus of human c-erbB-2/HER-2 protein. Species Sequence Homology: Rat: 100%. Cellular Localization: Cell membrane.
