Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.5-1ug/ml detected histone H3 on 10ug of Colemid treated HeLa acid extract.
Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B.
Proteins were visualized using an IgG (HRP) goat anti-rabbit secondary antibody and a chemiluminescence detection system
Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate
Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after recei.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Dr Hu Th Xe
Immunogen：Linear peptide coresponding to human Histone H3 at Ser10
