84-1016-41　cdk1, aa263-287 (cdc2 Kinase, Cyclin-dependent Kinase 1) 200ul　C2571-06

特徴

• Entry of all eukaryotic cells into mitosis is regulated by activation of CDC2 kinase.
• Activation of CDC2 is controlled at several steps including cyclin binding and phosphorylation of Thr161.
• However, the critical regulatory step in activating CDC2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14.
• Phosphorylation at Tyr15 and inhibition of CDC2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of CDC2 is carried out by the CDC25 phosphatase.
• Expression of CDC2 plays a critical role in cell transition through the G2/M phase.
• The activity of CDC2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation.
• Several proteins including Histone H1 and pp60src are substrates for CDC2.
• Applications:Suitable for use in ELISA, Western Blot, and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1:250-1:1000 detects cdk1/cdc2 in RIPA lysates from 3T3 cells.
• 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C2571-06 (1:2000).
• Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
• Immunoprecipitation: 10ul immunoprecipitates cdk1/cdc2 from 500ug of 3T3 RIPA lysate.
• Optimal dilutions to be determined by the researcher.
• Positive Antigen Control:3T3 cell lysate; use 20ug per lane for minigels.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.