Add 2.5µl of 2-mercaptoethanol and boil for five minutes prior to use.
Load 20µg per lane for minigels.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot 0.5–2.0ug/ml detected S6 Kinase in RIPA lysates from mouse 3T3 cells.
Additional bands above 64kDa may be observed at higher concentrations of the antibody.
Previous lots detected S6 Kinase in RIPA lysates from human A431 and rat L6 cells.
3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (1ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes rat p70 S6 kinase at 64kD. Species crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：16 residue synthetic peptide (C-FPMISKRPEHLRMNL) corresponding to residues 511- 525 of rat S6 kinase. The immunizing peptide is identical to the human and rabbit sequence.
